{"id":190,"date":"2023-08-21T07:58:59","date_gmt":"2023-08-21T07:58:59","guid":{"rendered":"http:\/\/ivfathenscenter.gr\/?page_id=190"},"modified":"2024-10-08T21:45:54","modified_gmt":"2024-10-08T21:45:54","slug":"fertilization","status":"publish","type":"page","link":"https:\/\/ivfathenscenter.gr\/en\/ivf\/fertilization\/","title":{"rendered":"Fertilization \/ Micro-insemination"},"content":{"rendered":"\r\n<section class=\"title-section \" >\r\n    <div class=\"ivf-container\">\r\n        <div class=\"page-title-wrap \">\r\n            <div>\r\n                <h1 class=\"h2 page-title\">FERTILIZATION \/ MICRO-INSEMINATION<\/h1>\r\n                            <\/div>\r\n        <\/div>\r\n    <\/div>\r\n<\/section>\r\n\n\n\n<section class=\" services-content\">\n\n    \n    <div class=\"ivf-container  ivf-small-container section-content-wrap split\">\n\n                    <div class=\"image \">\n                <img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"1000\" src=\"https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-1.png\" class=\"attachment-large size-large\" alt=\"\" srcset=\"https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-1.png 1000w, https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-1-300x300.png 300w, https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-1-150x150.png 150w, https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-1-768x768.png 768w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/>            <\/div>\n        \n        <div class=\"text\">\n            <p class=\"text__desc\">\n                <p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/GaZ2YAvPBXg?si=sYsB8spHQvDTVToo\" width=\"484\" height=\"273\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400\">In Vitro Fertilization (IVF) is the most widely known method in the area of Medically Assisted Reproduction and involves the fertilization of the ovum (egg) and the incubation of the embryo that are produced in laboratory conditions. The process is separated into various stages that start by investigating the existing fertility through a complete testing of both the female and the male factors as well as blood and hormonal check-up. Next comes the ovarian stimulation through medication that leads to embryo transfer, which is in fact, the completion of the procedure.<\/span><\/p>\n<p><span style=\"font-weight: 400\">Fertilization of the oocytes is either done with the method of classic In Vitro Fertilization (conventional IVF) or with the method of Intra-Cytoplasmic Sperm Injection (ICSI)<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><strong><span style=\"color: #b02e85\">Fertilization<\/span><\/strong><\/p>\n<ul>\n<li><strong><span style=\"color: #b02e85\">Conventional IVF<\/span><\/strong><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400\">Conventional IVF\u00a0 is the most widely known and the first used method in the area of Medically Assisted Reproduction. That involves the co-incubation of the oocyte\/s with sufficient number of specially prepared, motile spermatozoa for a period of 16-18 hours. Thus, the most progressive motile and usually normal forms of spermatozoa penetrate the zona of the oocytes, as it happens in natural conception, and fertilization occurs.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400\">After the co-incubation period (16-18 hours) the embryologists identify the properly fertilized oocytes and the embryonic life begins, under special conditions in special designed incubations.<\/span><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li><strong><span style=\"color: #b02e85\">ICSI ( Intracytoplasmic Sperm Injection)<\/span><\/strong><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/1351601\/\" target=\"_blank\" rel=\"noopener\">ICSI<\/a> is the most revolutionary method in IVF, since its\u00a0 accidental start in 1992 by Palermo in Brussels<\/span><span style=\"font-weight: 400\">. With this method, millions of children have been born worldwide, from their own father genetic material, even in cases with extremely low semen parameters.\u00a0\u00a0<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400\">For the procedure, the oocytes after the retrieval are isolated from the\u00a0 surrounding cells (cumulus mass cells) and only those which are mature (Mataphase II) are forwarded for micro-insemination.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400\">In a micromanipulation system, one spermatozoon\u00a0 is selected and aspirated into a special micropipette and inserted into each oocyte&#8217;s cytoplasm. The inseminated oocyte is then kept in culture conditions in an incubator and 16-18 hours later, embryologists check the evidence of two pronuclei in the cytoplasm (female and male) indicating the successful insemination technique. Further in vitro culture conditions are exactly the same as in conventional IVF. <\/span><\/p>\n            <\/p>\n        <\/div>\n\n        \n    <\/div>\n\n        \n<\/section>\n\n\n\n<section class=\" services-content services-content-2\">\n\n    \n    <div class=\"ivf-container  ivf-small-container section-content-wrap split\">\n\n        \n        <div class=\"text\">\n            <p class=\"text__desc\">\n                <ul>\n<li><strong><span style=\"color: #b02e85\">Improvements of ICSI: IMSI \/ PICSI (Physiological Intracytoplasmic Sperm Injection)<\/span><\/strong><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong><span style=\"color: #b02e85\"><b>PICSI (Physiological Intracytoplasmic Sperm Injection) Method<\/b><\/span><\/strong><br \/>\n<span style=\"font-weight: 400\">It is a way of fertilization based on the sperm\u2019s and oocyte\u2019s physiology, hence the name Physiological ICSI (PICSI).<\/span><\/p>\n<p><span style=\"font-weight: 400\"><br \/>\n<\/span><span style=\"font-weight: 400\">Its difference with ICSI is in the use of special plates and culture mediums where small quantities of processed sperm sample of the husband are placed.<\/span><span style=\"font-weight: 400\"><br \/>\n<\/span><span style=\"font-weight: 400\">In this material the binding of immature sperm forms is impossible because they do not have the suitable receptors. On the contrary, spermatozoa with distinctly limited chromosome aberrations and significant improvement of their nucleus\u2019 maturation are bound and selected for fertilization.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><strong><span style=\"color: #b02e85\"><b>IMSI (<\/b><b>Intracytoplasmic Morphologically Sperm injection) Method<\/b><\/span><\/strong><\/p>\n<p><span style=\"font-weight: 400\">It is an improvement of the technique of Micro-insemination (ICSI) where morphologically selected spermatozoa are injected during the fertilization.<\/span><span style=\"font-weight: 400\"><br \/>\n<\/span><span style=\"font-weight: 400\"><br \/>\n<\/span><span style=\"font-weight: 400\">It is used in cases of severe astheno-terato-spermia where in the man\u2019s sperm sample there are many spermatozoa with deviant morphology. With the help of special lenses high definition microscopy is achieved as well as enlargement of the image of the spermatozoa at about 6500 times. Therefore, we have detailed illustration of their head and morphology and only the best spermatozoa\u00a0 in terms of morphology are used for the oocyte insemination. Disadvantages of the method are that it is time consuming, not easily applied and doesn&#8217;t seem to be very sufficient.<\/span><\/p>\n<p>&nbsp;<\/p>\n            <\/p>\n        <\/div>\n\n                    <div class=\"image \">\n                <img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"1000\" src=\"https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-2.png\" class=\"attachment-large size-large\" alt=\"\" srcset=\"https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-2.png 1000w, https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-2-300x300.png 300w, https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-2-150x150.png 150w, https:\/\/ivfathenscenter.gr\/wp-content\/uploads\/2023\/08\/fertilization-2-768x768.png 768w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/>            <\/div>\n        \n    <\/div>\n\n        \n<\/section>\n\n\n    <section class=\"services-faqs reverse\" id=>\r\n\r\n                    <div class=\"ivf-container ivf-small-container section-title-wrap\">\r\n                <h3 class=\"section-title\">\r\n                    FAQs About Fertilization method                <\/h3>\r\n            <\/div>\r\n        \r\n        <div class=\"ivf-container ivf-small-container section-content-wrap\">\r\n            <div class=\"text\">\r\n                <div class=\"faq\">\r\n\r\n                                            <h4 class=\"faq-title active\">\r\n                            When is ICSI the first choice technique for fertilization?                        <\/h4>\r\n                        <div class=\"faq-desc\">\r\n                            <span class=\"faq-desc-content\">\r\n                                <p><span style=\"font-weight: 400\">Some indications for the ICSI procedure may are upon to male, female factors or combination of both:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Impaired semen parameters (low concentration and\/or motility, very many abnormal forms, presence of oxidative stress etc)\u00a0<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Presence of various cells in the sperm, cause of chronic inflammation<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Specific medical history (i.e. PGT cycles)<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Warming and fertilization of previously vitrified oocytes<\/span><\/li>\n<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Many failed previous IVF attempts , with\/and low impact<\/span><\/li>\n<\/ul>\n                            <\/span>\r\n                        <\/div>\r\n                                            <h4 class=\"faq-title \">\r\n                            Which technique between PICSI or IMSI proved to have better results and be more successful?                         <\/h4>\r\n                        <div class=\"faq-desc\">\r\n                            <span class=\"faq-desc-content\">\r\n                                <p><span style=\"font-weight: 400\">In each technique (PICSI or IMSI) we used either separately or in combination, we select mature and morphologically normal spermatozoa.<\/span><span style=\"font-weight: 400\"><br \/>\n<\/span><span style=\"font-weight: 400\"><br \/>\n<\/span><span style=\"font-weight: 400\">Especially for the PICSI method, which is used extensively in the IVF Athens Center, there is distinct improvement of the embryo quality, increased chance of implantation, increase on the rate of clinical pregnancies and decrease of the clinical miscarriages.<\/span><\/p>\n                            <\/span>\r\n                        <\/div>\r\n                    \r\n                <\/div>\r\n            <\/div>\r\n        <\/div>\r\n    <\/section>\r\n\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":6297,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-190","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/pages\/190","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/comments?post=190"}],"version-history":[{"count":60,"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/pages\/190\/revisions"}],"predecessor-version":[{"id":6075,"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/pages\/190\/revisions\/6075"}],"up":[{"embeddable":true,"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/pages\/6297"}],"wp:attachment":[{"href":"https:\/\/ivfathenscenter.gr\/en\/wp-json\/wp\/v2\/media?parent=190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}